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J Korean Soc Environ Eng > Volume 41(8); 2019 > Article
J Korean Soc Environ Eng 2019;41(8): 419-430. doi: https://doi.org/10.4491/KSEE.2019.41.8.419
접촉 작동식 기기(승강기 버튼, 현금인출기, 스마트폰) 표면에 존재하는 미생물의 분리, 계수 및 동정
김지은, 이은빈, 양윤정, 김우형, 오정민, 임대용, 박예닮, 최민석, 이채영, 안연수, 송학진, 윤재윤, 김형주
건국대학교 생물공학과
Isolation, Counting and Identification of Microorganisms from Elevator Button, ATM, and Smartphone Surface
Ji Eun Kim, Eun Bin Lee, Yun Jeong Yang, Woo Hyeong Kim, Jung Min Oh, Dae Yong Lim, Ye Darm Park, Min Suk Choi, Chae Young Lee, Yeon Soo An, Hak Jin Song, Yoon Jae Yoon, Hyung Joo Kim
Department of Biological Engineering, Konkuk university
Corresponding author  Hyung Joo Kim ,Tel: 02-2049-6111, Fax: 02-3437-8360, Email: hyungkim@konkuk.ac.kr
Received: June 13, 2019;  Revised: July 23, 2019;  Accepted: July 25, 2019.  Published online: August 31, 2019.
ABSTRACT
Objectives:
The purpose of this study was to identify and isolate bacteria on the surface of elevator buttons, ATM screens, and smartphone surfaces. Through this process, bacterial diversity and presence of pathogens on the surface were evaluated for the prospective public health and environment.
Method:
Bacterial samples collected from elevator buttons, ATM screen surfaces, and smartphone surfaces were analyzed their CFUs using LB agar plates. Unique colonies in the plates were isolated and identified by the 16S rRNA analysis. The diversity of the bacteria in the collected samples containing high number of CFU was monitored using the Denaturing Gradient Gel Electrophoresis (DGGE).
Results and Discussion:
Among the instrument surfaces, the elevator buttons showed the highest CFU (average: 163±17 CFU/cm2). The samples from smartphone and ATM surfaces have 66±13 and 6±1 CFU, respectively. The CFU analysis also showed that the all collected samples had active bacterial species, and the 16S rRNA analysis indicated that pathogenic bacteria were existed on the surface on the buttons and screens. The DGGE analysis showed that higher diversity of bacterial species were found compare to the CFU analysis of the samples. This difference in the bacterial diversity is probably due to the difference in optimal cultivation conditions of each bacterial species in the samples.
Conclusions:
The user interfaces of the elevators, ATMs, and smartphones contained high diversity of normal and pathogenic bacterial species. Thus attention must be paid to the transfer of pathogenic bacteria in children, pregnant women, immunocompromised patients.
Key Words: Elevator button, Smartphone surface, ATM screen, Colony forming unit, DGGE
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